Cryogenic Stopped Flow.
Uses cryogenics to slow down rapid kinetics processes.
Cryo stopped-flow covers temperature ranges below -15°C where traditional stopped-flow experiments are limited. Cryo stopped-flow instruments are now well-established systems in inorganic and bio-inorganic chemistry laboratories where they have proved to be efficient tools used to identify reaction intermediates and define reaction mechanisms.
Conventional stopped-flow systems are usually limited to -15°C/-20°C because of the materials used in valves and driving syringes. However, some chemical reactions are still too fast to be observed at these sub-zero temperatures. One solution to elucidate the reaction mechanism is to slow down the reaction. By reducing the reaction temperature to -90°C the reaction becomes about 10,000 times slower when compared to ambient temperature, meaning that some events become observable. Cryo stopped-flow covers temperature ranges below -15°C where traditional stopped-flow experiments are limited. Cryo stopped-flow instruments are now well-established systems in inorganic and bio-inorganic chemistry laboratories where they have proved to be efficient tools used to identify reaction intermediates and define reaction mechanisms.
Cryogenic option down to -90°C
The cryogenic option extends the range of Bio-Logic stopped-flow to -90°C. It is available for single and double mixing applications. The mixers, the cuvette and the transfer lines are immersed in a cryo-solvent. Two cooling methods are available for the cryo bath: using liquid N2 circulation or using a commercial cryostat. Temperature dependence studies can be carried out from -90°C to +20°C without any reconfiguration. The mixing chamber is connected to the detection system using fiber optics and spectra can be recorded every 400 µs using our fast diode array detectors to fully benefit from the 2ms dead time.
Bio-Kine is user-friendly software that controls the stopped-flow, the detection device, follows the acquisition, and analyzes data.
Single mixing applications
Two levels of operation are provided for mixer control. The first level is designed for rapid and easy experiment design. The user sets the mixing ratio, the size of the cuvette and the volume of samples. Color coded windows display calculated values and alert the user to out of range conditions. The estimated dead time is automatically displayed, and a pre-trigger is also available to be sure the stationary state is reached accurately. This interface is ideal for routine mixing designs, occasional users, or students.
Cryostat or Liquid Nitrogen cooling
Single and double mixing
Temperature range : -90°C to +20°C
Dead time : 2 ms
Mixing ratio: 1:1 to 1:5
Stopped-flow in cryogenic conditions – SFM-2000 series – Rapid kinetics – Application Note 25
Stopped-flow in cryogenic conditions using an external cryostat – Rapid Kinetics – Application Note 25b
Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis
Solution behavior of iron(III) and iron(II) porphyrins in DMSO and reaction with superoxide. Effect of neighboring positive charge on thermodynamics, kinetics and nature of iron-(su)peroxo product
Nitrogen Oxide Atom-Transfer Redox Chemistry; Mechanism of NO(g) to Nitrite Conversion Utilizing μ-oxo Heme-FeIII–O–CuII(L) Constructs
The following topic is relevant to the Cryogenic Stopped-flow...