Spectroscopy solutions for life sciences
Wide product range: Circular Dichroism (CD), High throughput CD Microplate spectropolarimeters and pump-probe spectrometers
Constant innovation in the field of optical spectroscopy is necessary to constantly break down scientific barriers and advance research programs. This is especially important in the field of Lifesciences where high-performance, high-precision, and high-speed spectrometers are essential. As a leader in this field, BioLogic has over 30 years of experience in developing equipment designed to give researchers a cutting-edge. Originally dedicated to rapid kinetics measurements, BioLogic now also develops, integrates, and manufactures a wide range of instrumentation for steady-state spectroscopy including spectropolarimeters.
Circular dichroism (CD) has become one of the major steady-state spectroscopy techniques. Mostly reserved to R&D and protein structural studies in the past it has now found use in new applications from far-UV to NIR fields in organic chemistry and in the pharmaceutical industry. CD is now recognized as an important tool for the identification of leads in drug discovery and as such, is a much faster alternative than HPLC for the identification of enantiomers.
Industry has placed new demands for high-throughput analyzers offering sensitivity and high-speed measurements. BioLogic offers solutions for traditional research-grade spectropolarimeter but also for screening, with the first CD microplate able to read thousands of wells per hour.
Rapid kinetics spectrometers
Spectrometers optimized for rapid kinetics are often associated with stopped-flow systems, a technique where two, three, or four solutions are mixed together. Researchers need to continuously follow the evolution of reactions in the mixing chamber at a millisecond timescale in order to understand the reaction mechanism or the structural properties of samples. They, therefore, need fast, accurate and high signal-to-noise detectors optimized for these demanding applications. BioLogic offers a wide range of optical solutions for these fast measurements: fluorescence, absorbance, light scattering, and polarization techniques such as circular dichroism and anisotropy. This need for fast and ultra-sensitive detectors is enhanced when studying photosynthesis antennas directly on leaves or algae, as the changes of the signal are microscopic. For such applications, the pump-probe spectroscopy approach should be the technique of choice.
The MOS Range of spectrometers: MOS200-MOS200M, MOS-500, MOS-DAD, MCS-200
A leader in the field of Rapid Kinetics, BioLogic has over 30 years of experience in designing and manufacturing state-of-the-art measurement instruments. With its origins born out of rapid kinetics instrument design and manufacture, BioLogic now also develops, integrates, and manufactures a wide range of instrumentation for steady-state spectroscopy including spectropolarimeters.
The MOS product range is made up of four key instruments:
- MOS-200, MOS-200M Dual absorbance fluorescence spectrometer for rapid kinetics
- MOS-500 Circular dichroism spectrometer
- MOS-DAD Fast diode array spectrometer
- MCS-200 (a fast conductimeter used as a stopped-flow detector)
MOS200 – MOS200M
Dual Absorbance/fluorescence spectrometer: MOS-200 and MOS-200/M
The MOS-200 is a simple, fast, and sensitive rapid kinetics spectrometer optimized for single wavelength absorbance and fluorescence. Combined with a stopped-flow, it is the perfect detector for single wavelength kinetics studies. Its 10 µs time resolution allows users to follow the fastest reaction ever observable by stopped-flow, with a 0.25 ms dead time. The same detector can be used for absorbance and fluorescence stopped-flow. The excitation monochromator can be set to manual or motorized mode to allow excitation scanning and wavelength tracking. The MOS-200 can also accommodate other detectors for FRET or fluorescence anisotropy measurements.
MOS 500 Circular Dichroism Spectrometer
The MOS-500 is a circular dichroism spectrometer developed to match all steady-state and kinetics applications. The MOS-500 spectrometer uses an innovative and patented three-stage wavelength selection system to bypass the limitations of traditional prism-based monochromators.
This innovative spectrometer design delivers outstanding performance in wavelength range, sensitivity, precision, speed, and modularity. Operating costs are also reduced as the MOS-500 requires optics purging only when working below 195 nm. A standard dual lamp box adds to the convenience of the spectrometer system, and an optional tungsten lamp enhances IR performance.
Thanks to its modular design, the MOS-500 is so much more than just a circular dichroism spectrometer. Multiple detection modes and a wide range of accessories from stopped-flow to ORD are available to allow you to adapt the system to your specific research needs.
Diode array detector: MOS-DAD
A range of high-speed diode arrays for spectral time-resolved acquisition. The diode array spectrometer collects spectra with a 400µs time resolution from UV to NIR, the MOS-DAD diode array detector is used intensively for capturing intermediates of reactions in stopped-flow applications. The diode array generates 3D data ( wavelength, time, absorbance) which can then be analyzed to extract single absorbance spectra of all reaction intermediates. The diode array spectrometer is also perfectly suited for spectro-electrochemistry when combining cyclic voltammetry with absorbance spectra measurements.
Conductimeter used as a stopped-flow detector
MCS-200 is an impedance potentiostat optimized for stopped-flow conductivity measurements. Conductivity is a useful technique when changes in ion concentrations are observed during chemical reactions. Conductivity detection is ideally suited to inorganic reduction/oxidation reaction, proton exchange, metal-ligand binding, and micelle formation from surfactants. Conductivity can generate complementary information to absorbance meaning the simultaneous acquisition of two signals is possible.
For more information about the MCS-200 please click here.
CD microplate reader: EKKO
The EKKO spectrometer is a circular dichroism microplate reader specifically optimized for chiral screening. The instrument represents a major breakthrough in automated screening for chiral pharmaceuticals or in automated circular dichroism measurements. EKKO’s high scanning speed enables it to identify hits faster, with fewer resources (human/energy), than standard CD instruments, making it a vital R&D tool for pharmaceutical and biotech companies or any academic institution needing to accelerate/optimize catalytic/asymmetric syntheses, quickly and efficiently. 96 single wavelength measurements can be performed in less than 2 minutes. EKKO is the alternative to chiral HPLC by reducing measurement time by a factor of 10 to 50, making circular dichroism the new reference technique for chiral screening.
Pump probe spectrometer JTS-150
The JTS-150 is a LED pump-probe spectrometer designed for electron transfer studies in photosynthetic organisms via fluorescence and absorbance changes. Up to 9 wavelengths can be simultaneously followed to provide a an overall understanding of the chemical reactions involved in photosynthesis. The optical bench maximizes the measurement of small signals even within strongly scattered samples such as leaves or cell suspensions. The JTS-150 provides fluorescence data that is analogous to pulsed-amplitude modulated (PAM) fluorometers and is thus well adapted to chlorophyll fluorescence and to photochemical quenching application. The JTS-150 is designed for versatility and custom assay developments.