Spectroscopy solutions for life sciences

 

Constant innovation in the field of optical spectroscopy is necessary to constantly break down scientific barriers and advance research programmes. This is especially important in the field of Lifesciences where high-performance, high-precision and high-speed spectrometers are essential. As a leader in this field, BioLogic  has over 30 years of experience of developing equipment designed to give researchers a cutting-edge. Originally dedicated to rapid kinetics measurements, BioLogic  now also develops, integrates and manufactures a wide range of instrumentation for steady state spectroscopy including spectropolarimeters.

 

Circular Dichroism

Circular dichroism (CD) has become one of the major steady-state spectroscopy techniques. Mostly reserved to R&D and protein structural studies in the past it has now found use in new applications from far-UV to NIR fields in organic chemistry and in the pharmaceutical industry. CD is now recognized as an important tool for the identification of leads in drug discovery and as such, is a much faster alternative than HPLC for the identification of enantiomers.

Industry has placed new demands for high-throughput analyzers offering sensitivity and high-speed measurements. BioLogic offers solutions for traditional research-grade spectropolarimeter but also for screening, with the first CD microplate able to read thousands of wells per hour.

 

Rapid kinetics spectrometers

Spectrometers optimized for rapid kinetics are often associated with stopped-flow systems, a technique where two, three or four solutions are mixed together. Researchers need to continuously follow the evolution of reactions in the mixing chamber at a millisecond timescale in order to understand the reaction mechanism or the structural properties of the sample. They, therefore, need fast, accurate and high signal-to-noise detectors optimized for these demanding applications. Bio-Logic offers a wide range of optical solutions for these fast measurements: fluorescence, absorbance, light scattering and polarization techniques such as circular dichroism and anisotropy. This need for fast and ultra-sensitive detectors is enhanced when studying photosynthesis antenna directly on leaves or algae, as the changes of the signal are microscopic. For such applications, the pump-probe spectroscopy approach should be the technique of choice.

Circular Dichroism MOS-500

A spectropolarimeter optimized for steady-state and rapid kinetics applications using proteins, peptides or other chiral material. The MOS-500 delivers outstanding performance in sensitivity, precision, speed and modularity spectroscopy from far UV to NIR region. The MOS-500 uses an innovative, patented three-stage wavelength selection system to bypass the limitation of prism-based monochromators. The MOS-500 is not only a CD spectrometer as it features in standard absorbance, excitation fluorescence, linear dichroism, and fluorescence anisotropy capabilities. A range of accessories and sample holders is also available to further exploit circular dichroism instruments.

 

 

CD microplate reader: EKKO

EKKO™ is the world’s first well plate reader designed for carrying out high-throughput circular dichroism measurements, making it an important tool for the pharmaceutical industry. The CD microplate reader can accommodate 96 or 384 well plates allowing thousands of readings per day. When compared to HPLC and conventional CD autosamplers, EKKO™ offers ten to hundreds of times higher throughput. 96 enantiomeric excess values can be read in less than 2 minutes.

 

Pump probe spectrometer JTS-150

The JTS-150 is a LED pump probe spectrometer designed for electron transfer studies in photosynthetic organisms via fluorescence and absorbance changes. The optical bench maximizes measurement of small signals even within strongly scattered samples such as leaves or cell suspensions. The JTS-150 is designed for versatility and custom assay developments.

 

Dual Absorbance/fluorescence spectrometer: MOS-200 and MOS-200/M

The MOS-200 is a simple, fast and sensitive rapid kinetics spectrometer optimized for absorbance and fluorescence. Combined to one of a stopped-flow it is the perfect detector for single wavelength kinetics studies. Its 10 µs time resolution allows users to follow the fastest reaction ever observable by stopped-flow with a 0.25 ms dead time. The excitation monochromator can be set to manual or motorized to allow excitation scanning and wavelength tracking. The MOS-200 can also accommodate other detectors for FRET or fluorescence anisotropy measurements.

 

Diode array detector: MOS-DAD

A range of high-speed diode arrays for spectral time-resolved acquisition. With a 400µs time resolution from UV to NIR, the MOS-DAD is used intensively for capturing intermediates of reactions in stopped-flow applications. It is also perfectly suited for spectro-electrochemistry when combining cyclic voltammetry with absorbance spectra measurements.

Impedance spectrometer: MCS-200

The MCS-200 is a conductivity detector with integrated impedance spectroscopy capability. Conductivity detection is an alternative to standard optical detection techniques when a chemical reaction induces a change in the ion concentration or a change of their mobility inside the solution. Simultaneous absorbance and conductivity measurements are possible with a 200µs time resolution. The impedance spectrometer can be used as a single potentiostat in standard for the cleaning of electrodes and for classical electrochemistry application.