Stopped-flow/quench flow

FT-IR Stopped-flow.

A valuable tool for evaluating reaction rates in Infra-Red regions.

Provides structural information in terms of bonds and mobility, the accessory can be used for infrared kinetics.

Provides structural information in terms of bonds and mobility, the accessory can be used for infrared kinetics.

Improvement in Fourier Transform Infra-Red (FT-IR) technology and the use of step scan acquisition have made coupling the FT-IR with stopped-flow instruments a valuable tool for evaluating reaction rates in Infra-Red regions. FT-IR spectroscopy provides structural information in terms of bonds and mobility. It can be a very useful accessory to study infrared kinetics in the amide I region of a protein’s vibrational spectrum.

Compatible with most FT-IR spectrometers

The FT-IR accessory is usually attached to stopped-flow using an umbilical connector so only the observation cuvette is fitted into FT-IR sample compartment. The FT-IR flow cell is mounted in a holder compatible with most FT-IR instruments.

 

The flow cell uses CaF2 windows and user can freely select light paths from 15 μm to 500 μm by changing a spacer between windows. Depending on the light path the total flow rate may be limited to 1 ml/s. The dead time under these conditions is determined by the acquisition speed of the detector.

FT-IR accessory includes:

  • FT-IR flow cell holder with built-in Berger Ball mixer,
  • Set of CaF2 windows,
  • Set of spacers,
  • Direct exit tube.

 

Specifications  

Window material: CaF2

Lightpath:15 μm, 25 μm, 50 μm, 100 μm, 200 μm, 500 μm

Dead time :(with 100 μm spacer and 3 ml/s flow rate)     6 ms

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