Application notes 5 min read

Which Quench Flow for my lab ? QFM & SFM Series – Quench flow – AN37

Latest updated: September 8, 2020

The chemical quench flow instrument, commonly called quench flow, is a member of the rapid kinetics instruments family. It is used to follow reactions on the milliseconds to seconds timescale. Depending on the application it can be used as an alternative to stopped-flow (for example when no optical detection is possible). Alternatively it can be used as a complement to stopped-flow (when you wish to trap an intermediate of reaction for structural analysis). In a quench flow instrument, the reaction is initiated by mixing two reactants. The solution is allowed to age in a delay line ( also called the ageing line or ageing loop) for a user-defined time before being stopped by a third sample (the quencher) in a second mixer. The solution is then collected for external analysis.

This note describes the different solutions offered by BioLogic to address chemical quench flow applications and the questions one should ask to identify the right instrument.

Quench flow microvolume QFM4000 SFM-3000 SFM-4000 rapid kinetics single mixing quench flow double mixing quench flow H/D exchange experiment radioactive labelling pulse flow continuous flow microvolume microvolume quench flow

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