Application notes 20 min read

Reproducibility of CD measurements – EkkO – Spectroscopy – Application Note 31

Latest updated: May 6, 2020

Introduction

EKKO™ CD Microplate Reader Reproducibility of CD measurements

The differential absorption between left and right circularly polarized light is a commonly used technique. Circular dichroism (CD) is often used for assigning the secondary structures of proteins and determining enantiomeric purities in asymmetric syntheses, both of which benefit from the ability to do the measurements in a high-throughput fashion.

The primary advantage of the EKKO™ CD Microplate Reader is its speed resulting from the use of well plates allowing for the highest throughput possible. It accomplishes this by turning the light path from the horizontal to vertical, allowing for the use of a computer-controlled XY stage so that CD signals are read directly from a well plate…

 

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Spectroscopy

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