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Insight into the binding interactions of fluorenone-pendent Schiff base with calf thymus DNA

Latest updated: November 21, 2023


Neha, Navneet Kaur
DOI: https://doi.org/10.1016/j.ab.2023.115216
Instrument(s) Used: MOS-500,


A novel fluorenone appended Schiff base (L) has been synthesized and utilized for studying the binding interactions with Calf Thymus DNA (ct-DNA). The mechanism of binding with ct-DNA was explored by employing various spectroscopic techniques viz. UV–Vis absorption spectroscopy, fluorescence emission spectroscopy, gel-electrophoresis, circular dichroism (CD), melting studies, viscosity arrays and molecular modeling methodology. The interpretation of UV–vis absorbance spectra pointed to binding of L within minor groove of ct-DNA with the binding constant of Kb = 0.15 × 104 M−1. Dye-displacement studies with Rhodamine-B (RhB) and Ethylene Bromide (EB) in fluorescence spectroscopy verified the groove binding mode of interaction between L and ct-DNA. Melting studies, circular dichroism, and viscosity studies further elucidated the binding modes of L with ct-DNA. Thermodynamic variable measurements taken at various temperatures such as ΔG, ΔH, and ΔS revealed that hydrophobic forces played a significant role in the binding process. The meticulous computational interaction demonstrated by molecular docking confirmed the minor groove binding of L with ct-DNA.


Circular dichroism DNA binding

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