Topic 10 min read

Automatic concentration dependence studies.

Latest updated: January 9, 2023

Save precious time and eliminate human error dilutions

Concentration dependence studies follow kinetic reactions whilst varying the concentration of one of the reagents. This series of concentration experiments is important to help scientists understand the order of the reaction or to build Chevron plots rapidly. However, these studies are laborious and time consuming when performed manually.

 

BioLogic stopped flow systems are designed so that a series of automatic concentration steps for a complete study, can be carried out quickly. In a couple of minutes with outstanding levels of precision, a series of concentration experiments can be completed 20 times more rapidly compared to rival instruments! The instruments also completely remove human dilution errors and minimize dead volume losses as the stopped-flow is only primed once, and not at each new concentration. Indeed, other stopped flows are unable to offer such performance due to poor instrument modularity and single drive mechanism.

 

Application Note #19 illustrates the use of the concentration dependence function of the SFM by following the concentration dependency of A in the reaction A + B, while keeping concentration B constant. This is carried out using 3 syringes and 2 mixers thanks to the independent stepping motor technology.

Stopped-flow automatic concentration dependence studies double mixing stopped-flow Chevron Plots independent stepping motors

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