The use of the SVP470 for Vibrating Probe measurements of plants – Scanning Probes. Application Note 22.

Abstract

With the Scanning Vibrating Electrode Technique (SVET) the extracellular currents of biological sample can be measured. The SVP470 was used to measure the backside of a spider plant leaf by SVET. SVET measurements show local current densities which correlate to the different features of the leaf.

Introduction

The SVP470 is the scanning vibrating probe module of the M470 scanning electrochemical workstation. Using the SVP470 it is possible to perform vibrating probe measurements to follow the local current distribution of a sample in solution.

Vibrating probe was first introduced by Nuccitelli and Jaffe in 1974 to investigate the extracellular currents associated with biological processes, such as embryo development [1]. Since this time vibrating probe has been applied to the investigation of plant photosynthesis [2, 3], wound healing [4], tumor cells [5], and limb regeneration [6]. Vibrating probe has also been used extensively in the related fields of corrosion and coatings. Vibrating probe was first introduced as the Scanning Vibrating Electrode Technique (SVET) to the field by Hugh Isaacs in the 1980s [7]….

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