Technical notes 5 min read

RK-Spectro Technical Note 80: Single wavelength kinetic using MOS-500 in fluorescence mode

Latest updated: February 17, 2021

Introduction

In this application note, we explain how to perform single wavelength kinetics using MOS-500 in fluorescence mode, a stopped-flow module, and Bio-Kine32. The scenario presupposes that the A/D board has been correctly installed and Bio-kine32 correctly configured. In a 6M GndHCl solution hen egg lysozyme is unfolded, the refolding can be followed by a 1:9 dilution with water.

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RK Spectro TN80 single wavelength kinetic using MOS 500 in fluorescence mode

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