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RK-Spectro Technical Note 63: Single wavelength kinetic of cytochrome c in fluorescence mode

Latest updated: May 6, 2020

Introduction

The objective of this technical note is to realize folding kinetics of cytochrome c at different temperatures: 25, 20, 15 and 10°C. Folding kinetics were initiate d by a 6-fold dilution of unfolded cytochrome c in 4.5M of Guanidine hydrochloride (pH 5.89 before mixing and final pH of 6.76 after folding).
The scenario documented presupposes a good understanding of the MOS-200 instrument. Bio-Kine version 4.52 was used to control the syringes, edit the sequences, follow the acquisition and to analyze results (fit calculation).

Related files

RK Spectro TN63 Single wavelength kinetic of cytochrome c in fluorescence mode

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