RK-Spectro Technical Note 42: Spectral recording using MOS450_AF-CD and Emission monochromator in fluorescence mode

Introduction

This Technical Note demonstrates how to perform an Emission fluorescence scan using MOS450/AF-CD, an emission monochromator, and Bio-Kine32 software. The MOS-450 /AF-CD is equipped with a 150W xenon lamp and 0.5 mm excitation slits are selected. The scenario presupposes that the A/D board has been correctly installed and Bio-kine32 software correctly configured. The scan is made between 300 nm and 500 nm, the excitation is carried out at 290 nm.