5 min read
RK-Spectro Technical Note 36: DNPA hydrolysis using the low temperature accessoryLatest updated: February 16, 2021
This Technical Note describes how to perform DNPA hydrolysis using the low-temperature accessory. The alkaline hydrolysis of 2,4-dinitrophenylacetate (DNPA) with sodium methoxide in methanol was used to demonstrate the possibilities of the instrument between +20°C and -80°C. The scenario presupposes that users understand how to operate the MOS-250 correctly. Bio-Kine version 4.0 was used to control the syringes, to edit the sequences, to follow the acquisition and to analyze the results
You cannot access this resource because you are not logged in. Log in
CD spectropolarimeter specifically optimized for both steady state and rapid kinetics applications. The CD spectrometer guarantees excellent performance: sensitivity, precision, speed and modularity from far-UV to NIR region.