RK-Spectro Technical Note 12: Single wavelength kinetics experiment using MOS450_AF or AF-CD in fluorescence mode

Introduction

The aim of this application note is to learn how to perform single wavelength kinetics using MOS450/AF (or /AF-CD) in fluorescence mode, a stopped-flow module, and Bio-Kine32. The A/D board is correctly installed and Bio-kine32 well configured. In a 6M GndHCl solution hen egg lysozyme is unfolded, the refolding can be followed by a 1:7 dilution with water.