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Meniscus and beam position effects on measurement stability – EkkO – Spectroscopy – Application Note 28

Latest updated: May 6, 2020

Introduction

Circular dichroism (CD), the commonly used technique for chiral analysis, refers to the differential absorption between left and right circularly polarized light. It is often used for assigning the secondary structures of proteins and determining enantiomeric purities in asymmetric syntheses, both of which require the ability to do the measurements in a high throughput fashion.
Traditional CD spectrometers use a horizontal light path that requires transferring the sample into a cuvette for measurement. Even though this process is expedited when robotic liquid handling systems are coupled to a conventional CD spectrometer, the cuvette must be cleaned between measurements. This is still a laborious and time-consuming process.

 

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