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Double mixing stopped-flow using interrupted flow method – SFM-2000 series – Rapid kinetics – Application Note 21

Latest updated: May 6, 2020

Abstract

Often, two reactants must be mixed then aged before mixing with a third reactant in kinetic analyses. The SFM-3000 and SFM-4000 are the ideal systems for these types of double mixing experiments taking full advantage of the precision of independent stepping motors. This application note illustrates the capabilities of the SFM 3000/4000 to mix reagents with variable aging times using the reduction of dichloroindophenol by ascorbic acid. No hardware reconfiguration is necessary because of the independent stepping motors are controlled by the software automatically, making the SFM instruments unique. All the user needs to do is define the mixing sequence and collect the data.

 

Introduction

SFM-3000 and SFM-4000 are the ideal systems for double mixing experiments taking full advantage of the precision of independent stepping motors. User can design easily a series of shots at different ageing times so reaction intermediates not visible with single mixing methods become observable.

Fig. 1: Double mixing set-up. In a double mixing experiment, two reactants are mixed in mixer 1 and are allowed to age during an user-defined ageing time. After that content of the delay line is mixed with a third reactant so this second reaction can be observed using optical method. Depending on the ageing time the mixing is done is continuous flow mode or in interrupted flow mode to cover an ageing time range from 2 ms to several minutes.

 

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