Application notes 8 min read

Getting the most from a 50 μl stock of sample using the μSFM for refolding experiments – μSFM – Rapid kinetics – Application Note 27

Latest updated: May 6, 2020

Reducing sample consumption in stopped-flow experiments has always been a high priority as the cost of sample is a major concern for the user. This application note demonstrates how the μSFM

Introduction

Reducing sample consumption in stopped-flow experiments has always been a high priority as the cost of sample (proteins, peptides…) can be hundreds or thousands of dollars per millilitre.
Typically for a refolding experiment (doing a 10 fold dilution) using a classical stopped-flow design the user would use 20-30 µl of sample per a single shot. So, considering you need to have about 9-10 shots for a good statistical analysis, and also considering the priming volume of the system, 500 µl is often considered as a minimum volume for your stock solution to schedule a series of experiments.
This application note demonstrates how the µSFM significantly reduces sample usage. Only 50 µl of precious sample would be required to plan a series of experiments!

 

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